CdaS

• Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
  
  

Gene name	cdaS
Synonyms	yojJ
Essential	no
Product	sporulation-specific diadenylate cyclase
Function	synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaS
Metabolic function and regulation of this protein in SubtiPathways: CdaS
MW, pI	22 kDa, 5.367
Gene length, protein length	621 bp, 207 aa
Immediate neighbours	yojK, yojI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU19430

Database entries

• BsubCyc: BSU19430

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • synthesis of c-di-AMP from two molecules of ATP PubMed

• Protein family:

• Paralogous protein(s): CdaA

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal autoinhibitory domain PubMed
  • C-terminal DAC domain involved in the synthesis of c-di-AMP PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms hexamers (enzymatically inactive) or dimers (active) PubMed
• Localization:

Database entries

• BsubCyc: BSU19430

• Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)

• UniProt: O31854

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cdaS PubMed

• Expression browser: cdaS PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP983 (cdaS::ermC), available in Jörg Stülke's lab
  • GP1360 (cdaS::spc), available in Jörg Stülke's lab

• Expression vector:
  • IPTG inducible expression of B. cereus Strep-cdaS in E. coli: pGP2593 (in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• His-tag fusion:
  • pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
  • pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab

• two-hybrid system:

• Antibody:

References

Original publications

Felix M P Mehne, Kathrin Schröder-Tittmann, Robyn T Eijlander, Christina Herzberg, Lorraine Hewitt, Volkhard Kaever, Richard J Lewis, Oscar P Kuipers, Kai Tittmann, Jörg Stülke
Control of the diadenylate cyclase CdaS in Bacillus subtilis: an autoinhibitory domain limits cyclic di-AMP production.
J Biol Chem: 2014, 289(30);21098-107
[PubMed:24939848] [WorldCat.org] [DOI] (I p)

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)