cdaS

cdaS
168

sporulation-specific diadenylate cyclase, synthesis of c-di-AMP

Locus
BSU_19430
Molecular weight
22.75 kDa
Isoelectric point
5.37
Protein length
207 aaSequenceBlast
Gene length
624 bpSequenceBlast
Function
synthesis of c-di-AMP
Product
sporulation-specific diadenylate cyclase
Essential
no
E.C.
2.7.7.85
Synonyms
cdaS, yojJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1624 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,118,504  2,119,127
Phenotypes of a mutant
a cdaA cdaS disA triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) PubMed
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-AMP from two molecules of ATP PubMed
2 ATP --> 3',3'-c-di-AMP + 2 diphosphate (according to UniProt)
Protein family
adenylate cyclase family (with CdaA, according to UniProt)
Domains
N-terminal autoinhibitory domain PubMed
C-terminal DAC domain involved in the synthesis of c-di-AMP PubMed
DAC domain (aa 63-205) (according to UniProt)
Structure
2FB5 (PDB) (the protein of B. cereus, 48% identity, 81% similarity)
O31854 (AlphaFold)
Paralogous protein(s)
CdaA
Expression and Regulation
Operons
Genes
cdaS
Description
PubMed
Regulation
expressed late during sporulation in the forespore (SigG) PubMed
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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cdaS

2025-02-15 12:11:51

ghost

71

46d5e4f41e0502899ea5aac5e84f0abec559ef9c

CE713202908833B1D3A579A1C3A2C86E345877E9

Biological materials
Mutant
MGNA-B426 (yojJ::erm), available at the NBRP B. subtilis, Japan
GP983 (''cdaS::ermC''), available in Jörg Stülke's lab PubMed
GP1360 (''cdaS::spc''), available in Jörg Stülke's lab
GP2222 (cdaA::cat cdaS::ermC ''disA::tet''), available in Jörg Stülke's lab, the mutant is only viable on minimal medium at low potassium concentration PubMed
BKE19430 (cdaS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC,  downstream forward: _UP4_TAAAAATAATAAAAAGGGCC
BKK19430 (cdaS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC,  downstream forward: _UP4_TAAAAATAATAAAAAGGGCC
Expression vectors
IPTG inducible expression of '''B. cereus' Strep-cdaS in E. coli'': pGP2593 (in pGP172), available in Jörg Stülke's lab
IPTG inducible expression of His-cdaS in E. coli: pGP1972 (in pET19B), available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
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Original Publications
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92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32

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Time of last update: 2025-03-31 22:00:54

Author of last update: Jstuelk