ThiO

• Description: FAD-dependent glycine oxidase
  
  

Gene name	thiO
Synonyms	goxB, yjbR
Essential	no
Product	glycine oxidase
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiO
Metabolic function and regulation of this protein in SubtiPathways: thiO
MW, pI	40 kDa, 5.898
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	tenI, thiS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11670

Database entries

• BsubCyc: BSU11670

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Glycine + H₂O + O₂ = glyoxylate + NH₃ + H₂O₂ (according to Swiss-Prot)

• Protein family: DAMOX/DASOX family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: FAD PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: the FAD-containing holoenzyme is a homotetramer PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11670

• Structure: 1NG3 (complex with acetyl-glycine), 1RYI

• UniProt: O31616

• KEGG entry: [3]

• E.C. number: 1.4.3.19

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: thiO PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 556 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1224 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1342 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1721 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 593 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: