thiF

thiF
168

hydroxyethylthiazole phosphate biosynthesis

Locus
BSU_11700
Molecular weight
36.24 kDa
Isoelectric point
5.62
Protein length
336 aaSequenceBlast
Gene length
1011 bpSequenceBlast
Function
biosynthesis of thiamine
Product
unknown
Essential
no
E.C.
null
Synonyms
thiF, yjbU
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0476 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,245,808 → 1,246,818
The protein
Catalyzed reaction/ biological activity
[sulfur-carrier protein ThiS]-C-terminal Gly-Gly + ATP + H+ --> [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + diphosphate (according to UniProt)
Protein family
HesA/MoeB/ThiF family (with MoeB and YrvM, according to UniProt)
Structure
1ZFN (PDB) (from E. coli, 35% identity, 51% similarity) PubMed
O31619 (AlphaFold)
Paralogous protein(s)
MoeB
Expression and Regulation
Operons
Genes
tenA-tenI-thiO-thiS-thiG-thiF-thiD
Description
PubMed
Regulation
repressed by thiamine (Thi-box) PubMed
antitermination is modulated by NusG-dependent transcription pausing just upstream of the Thi-box PubMed
the Thi-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
Thi-box: RNA switch, via RNA switch, in Thi-box regulon
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tenA→thiD

2025-03-13 14:23:12

Jstuelk

126

A48634F4963321F6F5F59C5107F472325227A1B0

A1FEA45B91FD65F137EB4D1A0FCD88BC91331EAF

Biological materials
Mutant
MGNA-B166 (yjbU::erm), available at the NBRP B. subtilis, Japan
BKE11700 (ΔthiF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCCTGCCTTGAATACCTGC,  downstream forward: _UP4_GGGTAATAAAAGGAGTGTAG
BKK11700 (ΔthiF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCCTGCCTTGAATACCTGC,  downstream forward: _UP4_GGGTAATAAAAGGAGTGTAG
References
Reviews
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Original Publications
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Page visits: 4377

Time of last update: 2025-04-02 02:01:23

Author of last update: Melvin.boenninger