tenA

tenA
168

thiaminase II

Locus
BSU_11650
Molecular weight
27.27 kDa
Isoelectric point
4.99
Protein length
236 aaSequenceBlast
Gene length
711 bpSequenceBlast
Function
thiamine salvage
Product
thiaminase II
Essential
no
E.C.
3.5.99.2
Synonyms
tenA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0819 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,242,449 1,243,159
The protein
Catalyzed reaction/ biological activity
4-amino-5-aminomethyl-2-methylpyrimidine + H2O --> 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH4+(according to UniProt)
H2O + thiamine --> 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole + H+ (according to UniProt)
Protein family
TenA family (single member, according to UniProt)
Structure
1YAF (PDB), 2QCX (PDB) (complex with formyl aminommethyl pyrimidine)
P25052 (AlphaFold)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
tenA-tenI-thiO-thiS-thiG-thiF-thiD
Description
PubMed
Regulation
repressed by thiamine (Thi-box) PubMed
antitermination is modulated by NusG-dependent transcription pausing just upstream of the Thi-box PubMed
the Thi-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
Thi-box: RNA switch, via RNA switch, in Thi-box regulon
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tenA→thiD

2025-03-13 14:23:12

Jstuelk

126

A48634F4963321F6F5F59C5107F472325227A1B0

A1FEA45B91FD65F137EB4D1A0FCD88BC91331EAF

Biological materials
Mutant
BKE11650 (tenA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAAATCATTCCCCCTCTG, downstream forward: _UP4_AATGTGGAGCTTCACGCCAT
BKK11650 (tenA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAAATCATTCCCCCTCTG, downstream forward: _UP4_AATGTGGAGCTTCACGCCAT
References
Reviews
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Original Publications
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47E47FB7D9C079DF603906694C4649A049814346

Page visits: 2836

Time of last update: 2025-04-02 10:26:17

Author of last update: Jstuelk