MntR

• Description: transcriptional regulator, (repression of mntH and mntA-mntB-mntC-mntD under high Mn(II) conditions)

Gene name	mntR
Synonyms	yqhN
Essential	no
Product	transcriptional regulator (DtxR family)
Function	regulation of manganese transport
Gene expression levels in SubtiExpress: mntR
Metabolic function and regulation of this protein in SubtiPathways: mntR
MW, pI	16 kDa, 5.631
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	yqhO, lipM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control, membrane proteins

The MntR regulon:

• mntA-mntB-mntC-mntD, mntH

The gene

Basic information

• Locus tag: BSU24520

Database entries

• BsubCyc: BSU24520

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mn(2+) acts as co-repressor (according to PubMed)

• Effectors of protein activity:

• Interactions:
  • active as dimer (according to PubMed)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU24520

• Structure: 2F5D (complex with manganese), 2HYG (apo-form)

• UniProt: P54512

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: mntR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 376 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 790 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Richard Brennan, Houston, Texas, USA Homepage

References

Original Publications

Amanda M McGuire, Bonnie J Cuthbert, Zhen Ma, Kristen D Grauer-Gray, Megan Brunjes Brophy, Kayce A Spear, Sumarin Soonsanga, Joseph I Kliegman, Sarah L Griner, John D Helmann, Arthur Glasfeld
Roles of the A and C sites in the manganese-specific activation of MntR.
Biochemistry: 2013, 52(4);701-13
[PubMed:23298157] [WorldCat.org] [DOI] (I p)

Misha V Golynskiy, William A Gunderson, Michael P Hendrich, Seth M Cohen
Metal binding studies and EPR spectroscopy of the manganese transport regulator MntR.
Biochemistry: 2006, 45(51);15359-72
[PubMed:17176058] [WorldCat.org] [DOI] (I p)

Mark A DeWitt, Joseph I Kliegman, John D Helmann, Richard G Brennan, David L Farrens, Arthur Glasfeld
The conformations of the manganese transport regulator of Bacillus subtilis in its metal-free state.
J Mol Biol: 2007, 365(5);1257-65
[PubMed:17118401] [WorldCat.org] [DOI] (P p)

Joseph I Kliegman, Sarah L Griner, John D Helmann, Richard G Brennan, Arthur Glasfeld
Structural basis for the metal-selective activation of the manganese transport regulator of Bacillus subtilis.
Biochemistry: 2006, 45(11);3493-505
[PubMed:16533030] [WorldCat.org] [DOI] (P p)

Misha V Golynskiy, Talib C Davis, John D Helmann, Seth M Cohen
Metal-induced structural organization and stabilization of the metalloregulatory protein MntR.
Biochemistry: 2005, 44(9);3380-9
[PubMed:15736948] [WorldCat.org] [DOI] (P p)

Scot A Lieser, Talib C Davis, John D Helmann, Seth M Cohen
DNA-binding and oligomerization studies of the manganese(II) metalloregulatory protein MntR from Bacillus subtilis.
Biochemistry: 2003, 42(43);12634-42
[PubMed:14580210] [WorldCat.org] [DOI] (P p)

Emmanuel Guedon, Charles M Moore, Qiang Que, Tao Wang, Rick W Ye, John D Helmann
The global transcriptional response of Bacillus subtilis to manganese involves the MntR, Fur, TnrA and sigmaB regulons.
Mol Microbiol: 2003, 49(6);1477-91
[PubMed:12950915] [WorldCat.org] [DOI] (P p)

Arthur Glasfeld, Emmanuel Guedon, John D Helmann, Richard G Brennan
Structure of the manganese-bound manganese transport regulator of Bacillus subtilis.
Nat Struct Biol: 2003, 10(8);652-7
[PubMed:12847518] [WorldCat.org] [DOI] (P p)

Emmanuel Guedon, John D Helmann
Origins of metal ion selectivity in the DtxR/MntR family of metalloregulators.
Mol Microbiol: 2003, 48(2);495-506
[PubMed:12675807] [WorldCat.org] [DOI] (P p)

Q Que, J D Helmann
Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins.
Mol Microbiol: 2000, 35(6);1454-68
[PubMed:10760146] [WorldCat.org] [DOI] (P p)