RocR

• Description: transcriptional activator of arginine utilization operons
  
  

Gene name	rocR
Synonyms
Essential	no
Product	transcriptional activator
Function	transcriptional activator of arginine utilization operons
Gene expression levels in SubtiExpress: rocR
Interactions involving this protein in SubtInteract: RocR
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	52.6 kDa, 6.08
Gene length, protein length	1383 bp, 461 amino acids
Immediate neighbours	rocD, yyzO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

• utilization of amino acids,

transcription factors and their control

• see also: glutamate metabolism

The gene

Basic information

• Locus tag: BSU40350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG

• Protein family: Sigma-54 interacting transcription activator

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • Sigma-54 factor interaction domain (143–372)
  • 2x nucleotid-binding domain (171–178),(233–242)
  • HTH motif (434–453)

• Modification:

• Cofactor(s): ornithine or citrulline are required for RocR-dependent transcription activation PubMed

• Effectors of protein activity:

• Interactions:
  • RocR-SigL

• Localization:

Database entries

• Structure:

• UniProt: P38022

• KEGG entry: [3]

Expression and regulation

• Operon: rocR (according to DBTBS)

• Expression browser: rocR PubMed

• Sigma factor: SigA PubMed

• Regulation: autoregulation by RocR PubMed

• Regulatory mechanism: autorepression

• Additional information:

Biological materials

• Mutant: QB5533 (aphA3), from Michel Debarbouille, available in Stülke lab
  • 1A911 ( rocR::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage