rocR

rocR
168

transcriptional activator of arginine utilization operons

Locus
BSU_40350
Molecular weight
52.01 kDa
Isoelectric point
6.08
Protein length
461 aaSequenceBlast
Gene length
1386 bpSequenceBlast
Function
transcriptional activator of arginine utilization operons
Product
transcriptional activator
Essential
no
Synonyms
rocR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3829 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,145,747 → 4,147,132
Phenotypes of a mutant
rocR is unable to use arginine as the sole nitrogen source, indicating that RocR is a positive regulator of arginine catabolism. PubMed
The protein
Catalyzed reaction/ biological activity
has ATPase activity: ATP + H2O --> ADP + H+ + phosphate PubMed
transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG PubMed
Protein family
Sigma-54 interacting transcription activator
Transcription factors activating transcription at SigL-dependent promoters
Domains
N-terminal PAS domain (aa 10-125) for effector (ornithine) binding PubMed
central Sigma-54 factor interaction domain and AAA+ ATPase domain (aa 143-372) PubMed
2x nucleotid-binding domain (171–178),(233–242)
HTH motif (434–453)
Cofactors
ornithine is required for ATPase activity and RocR-dependent transcription activation PubMed
Structure
1OJL (PDB) (from Salmonella typhimurium, corresponds to aa 143 ... 454, 41% identity) PubMed
P38022 (AlphaFold)
Effectors of protein activity
ornithine is required for ATPase activity and RocR-dependent transcription activation PubMed
ATP binding and hydrolysis are required for the formation of the transcriptionally active hexamers PubMed
Expression and Regulation
Operons
Genes
rocR
Description
PubMed
Regulation
autoregulation by RocR 7540694 PubMed
Regulatory mechanism
RocR: autorepression, PubMed, in rocR regulon
LexA: autorepression, in lexA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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rocR

2025-03-29 20:55:18

Jstuelk

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Biological materials
Mutant
GP2312 (ΔrocR::tet, available in Jörg Stülke's lab)
QB5533 (aphA3), from Michel Debarbouille, available in Jörg Stülke's lab
1A911 ( rocR::kan), PubMed, available at BGSC
BKE40350 (ΔrocR::erm, available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
BKE40350 (ΔrocR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTATGAACCTCCCTCA,  downstream forward: _UP4_CAATATCGGCTGAAGAAATT
BKK40350 (ΔrocR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTATGAACCTCCCTCA,  downstream forward: _UP4_CAATATCGGCTGAAGAAATT
Expression vectors
pGP3855: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab PubMed
pGP3856 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
FLAG-tag construct
GP4091 rocR-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
References
Reviews
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Original Publications
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Time of last update: 2025-04-02 10:31:48

Author of last update: Jstuelk