TenI

• Description: thiazole tautomerase
  
  

Gene name	tenI
Synonyms
Essential	no
Product	thiazole tautomerase
Function	biosynthesis of thiamine
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	22 kDa, 8.132
Gene length, protein length	615 bp, 205 aa
Immediate neighbours	tenA, thiO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: TMP-PPase family (according to Swiss-Prot)

• Paralogous protein(s): ThiE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1YAD

• UniProt: P25053

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: tenI PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Tadhg P Begley, Steven E Ealick, Fred W McLafferty
Thiamin biosynthesis: still yielding fascinating biological chemistry.
Biochem Soc Trans: 2012, 40(3);555-60
[PubMed:22616866] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)

Original publications

Amrita B Hazra, Ying Han, Abhishek Chatterjee, Yang Zhang, Rung-Yi Lai, Steven E Ealick, Tadhg P Begley
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.
J Am Chem Soc: 2011, 133(24);9311-9
[PubMed:21534620] [WorldCat.org] [DOI] (I p)

Angela V Toms, Amy L Haas, Joo-Heon Park, Tadhg P Begley, Steven E Ealick
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.
Biochemistry: 2005, 44(7);2319-29
[PubMed:15709744] [WorldCat.org] [DOI] (P p)

A S Pang, S Nathoo, S L Wong
Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.
J Bacteriol: 1991, 173(1);46-54
[PubMed:1898926] [WorldCat.org] [DOI] (P p)