BslA

• Description: amphiphilic protein, forms water-repellent surface layer of the biofilm
  
  

Gene name	bslA
Synonyms	yuaB
Essential	no
Product	biofilm surface layer
Function	biofilm formation
MW, pI	19 kDa, 9.987
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	gbsR, ktrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, DegU regulon

The gene

Basic information

• Locus tag: BSU31080

Phenotypes of a mutant

• reduced colony complexity PubMed
• loss of surface repellency of the biofilm PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YweA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed
  • biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
  • forms a layer on the biofilm surface PubMed

Database entries

• Structure:

• UniProt: P71014

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: bslA PubMed

• Expression browser: yuaB PubMed

• Sigma factor:

• Regulation:
  • expression is down-regulated in a rok mutant (indirect effect) PubMed

• Regulatory mechanism:
  • DegU-P: transcription activation PubMed, this might be indirect PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Akos T Kovács, Jordi van Gestel, Oscar P Kuipers
The protective layer of biofilm: a repellent function for a new class of amphiphilic proteins.
Mol Microbiol: 2012, 85(1);8-11
[PubMed:22607588] [WorldCat.org] [DOI] (I p)

Original publications

Kazuo Kobayashi, Megumi Iwano
BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms.
Mol Microbiol: 2012, 85(1);51-66
[PubMed:22571672] [WorldCat.org] [DOI] (I p)

Akos T Kovács, Oscar P Kuipers
Rok regulates yuaB expression during architecturally complex colony development of Bacillus subtilis 168.
J Bacteriol: 2011, 193(4);998-1002
[PubMed:21097620] [WorldCat.org] [DOI] (I p)

Daniel T Verhamme, Ewan J Murray, Nicola R Stanley-Wall
DegU and Spo0A jointly control transcription of two loci required for complex colony development by Bacillus subtilis.
J Bacteriol: 2009, 191(1);100-8
[PubMed:18978066] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)