SinR

• Description: transcriptional regulator of post-exponential-phase responses genes
  
  

Gene name	sinR
Synonyms	sin, flaD
Essential	no
Product	transcriptional regulator of post-exponential-phase responses genes
Function	control of biofilm formation
Metabolic function and regulation of this protein in SubtiPathways: Biofilm, Central C-metabolism
MW, pI	12 kDa, 7.177
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	sinI, tasA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biofilm formation, transcription factor, Spo0A regulon

Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repressor of biofilm genes, acts as co-repressor for SlrR PubMed

• Protein family:

• Paralogous protein(s): SlrR

Genes controlled by SinR

• Activation by SinR: comK

• Repression by SinR: aprE, epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed,kinB, rapG PubMed, sigD, spo0A,spoIIAA-spoIIAB-sigF, spoIIE, spoIIGA-sigE-sigG, spoVG PubMed, yqxM-sipW-tasA PubMed, yvgN PubMed, ywbD PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: SinI and SlrA are antagonists to SinR PubMed, SlrR is also antagonist of SinR PubMed

• Interactions: SlrA-SinR PubMed, SlrR-SinR PubMed, SinR-ScoC, SinR-SinI

• Localization:

Database entries

• Structure: 1B0N (complex SinR-SinI)

• UniProt: P06533

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • sinI-sinR PubMed
  • sinR PubMed

• Sigma factor:
  • sinI: SigA PubMed
  • sinR: SigA PubMed

• Regulation:
  • sinI: repressed under conditions that trigger sporulation (Spo0A) PubMed
  • sinI: repressed during exponential growth (ScoC) PubMed
  • sinI: repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: TMB079 sinR::spec, GP736 (tetR), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP960 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

References

Reviews

Patrick Piggot
Epigenetic switching: bacteria hedge bets about staying or moving.
Curr Biol: 2010, 20(11);R480-2
[PubMed:20541494] [WorldCat.org] [DOI] (I p)

David Dubnau
Swim or chill: lifestyles of a bacillus.
Genes Dev: 2010, 24(8);735-7
[PubMed:20395361] [WorldCat.org] [DOI] (I p)

Modelling of the SinI/SinR switch

Jennifer S Hallinan, Goksel Misirli, Anil Wipat
Evolutionary computation for the design of a stochastic switch for synthetic genetic circuits.
Annu Int Conf IEEE Eng Med Biol Soc: 2010, 2010;768-74
[PubMed:21095906] [WorldCat.org] [DOI] (P p)