SinR

• Description: transcriptional regulator of post-exponential-phase responses genes
  
  

Gene name	sinR
Synonyms	sin, flaD
Essential	no
Product	transcriptional regulator of post-exponential-phase responses genes
Function	regulation of post-exponential gene expression
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	12 kDa, 7.177
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	sinI, tasA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): SlrR

Genes controlled by SinR

• Activation by SinR: comK

• Repression by SinR: aprE, kinB, rapG PubMed, sigD, spo0A,spoIIAA-spoIIAB-sigF, spoIIE, spoIIGA-sigE-sigG, spoVG PubMed, yqxM-sipW-tasA PubMed, yvgN PubMed, ywbD PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: SinI and SlrA are antagonists to SinR PubMed

• Interactions: SlrA-SinR PubMed, SinR-ScoC, SinR-SinI

• Localization:

Database entries

• Structure: 1B0N (complex SinR-SinI)

• UniProt: P06533

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during exponential growth (ScoC) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: TMB079 (spc), available in the Thorsten Mascher lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: