ThiO

• Description: FAD-dependent glycine oxidase
  
  

Gene name	thiO
Synonyms	goxB, yjbR
Essential	no
Product	glycine oxidase
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiO
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	40 kDa, 5.898
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	tenI, thiS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11670

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Glycine + H₂O + O₂ = glyoxylate + NH₃ + H₂O₂ (according to Swiss-Prot)

• Protein family: DAMOX/DASOX family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: FAD PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: the FAD-containing holoenzyme is a homotetramer PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1NG3 (complex with acetyl-glycine), 1RYI

• UniProt: O31616

• KEGG entry: [3]

• E.C. number: 1.4.3.19

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: thiO PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)