PksC

• Description: bacillaene synthase trans-acting acyltransferase
  
  

Gene name	pksC
Synonyms
Essential	no
Product	bacillaene synthase trans-acting acyltransferase
Function	polyketide (bacillaene) synthesis
Gene expression levels in SubtiExpress: pksC
MW, pI	32 kDa, 5.328
Gene length, protein length	864 bp, 288 aa
Immediate neighbours	pksB, pksD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

AbrB regulon, CodY regulon

The gene

Basic information

• Locus tag: BSU17100

Database entries

• BsubCyc: BSU17100

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU17100

• Structure:

• UniProt: O34825

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR PubMed

• Expression browser: pksC PubMed

• Sigma factor:

• Regulation:
  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • CodY: transcription activation PubMed

• Additional information:
  • this is a very large operon comprising about 75 kb
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 100 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Carol Vargas-Bautista, Kathryn Rahlwes, Paul Straight
Bacterial competition reveals differential regulation of the pks genes by Bacillus subtilis.
J Bacteriol: 2014, 196(4);717-28
[PubMed:24187085] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Fiona Cuskin, Alexandra S Solovyova, Richard J Lewis, Paul R Race
Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2011, 67(Pt 4);464-6
[PubMed:21505242] [WorldCat.org] [DOI] (I p)