pksI

pksI
168

involved in polyketide synthesis

Locus
BSU_17170
Molecular weight
27.80 kDa
Isoelectric point
5.61
Protein length
249 aaSequenceBlast
Gene length
750 bpSequenceBlast
Function
polyketide synthesis
Product
unknown
Essential
no
Synonyms
pksI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1024 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,792,012 → 1,792,761
The protein
Protein family
enoyl-CoA hydratase/isomerase family (according to UniProt)
Structure
4Q1G (PDB) and 4Q1H (PDB) (wildtype with different buffer components co-crystallized)
4Q1I (PDB) (A80K)
4Q1J (PDB) (A230H)
4Q1K (PDB) (A232K)
P40802 (AlphaFold)
Expression and Regulation
Operons
Genes
pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
Description
PubMed
Regulation
expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
CodY: activation, PubMed, in codY regulon
Additional information
this is a very large operon comprising about 75 kb
Open in new tab

pksC→pksR

2025-03-17 18:36:51

Jstuelk

162

74BF817D9000509D225BBBCAC6C8A0A9EABEB843

1475C94AAA2C10C6BE3CA0873EFBD171EC2E2513

Genes
pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
Description
PubMed
Open in new tab

pksB→pksR

2025-03-17 03:33:23

Jstuelk

136

c851e07b7201353cf026d8929ea66cff6bbb92e2

D95CF77A7673AA84CC653095A1FB017D490049D6

Biological materials
Mutant
BKE17170 (ΔpksI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTCCTCCTAACGGT,  downstream forward: _UP4_TAACTGAAAATATATATAAA
BKK17170 (ΔpksI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTCCTCCTAACGGT,  downstream forward: _UP4_TAACTGAAAATATATATAAA
References
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F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B

Page visits: 2485

Time of last update: 2025-04-02 02:01:23

Author of last update: Melvin.boenninger