EpsA

• Description: extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation
  
  

Gene name	epsA
Synonyms	yveK
Essential	no
Product	unknown
Function	biofilm formation
Gene expression levels in SubtiExpress: epsA
Interactions involving this protein in SubtInteract: EpsA
Regulation of this protein in SubtiPathways: epsA
MW, pI	25 kDa, 6.071
Gene length, protein length	702 bp, 234 aa
Immediate neighbours	epsB, slrR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, biofilm formation, membrane proteins

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU34370

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cpsC/capA family (according to Swiss-Prot)

• Paralogous protein(s): TkmA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • EpsA-EpsB (based on TkmA-PtkA interaction)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P71050

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

• Expression browser: epsA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription anti-activation (prevents binding of RemA) PubMed
  • RemA: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
  • the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed

Biological materials

• Mutant:
  • GP1517 (aphA3), available in Stülke lab
  • GP1519 (epsAB, aphA3), available in Stülke lab
  • GP1567 epsA::aphA3 tkmA::spc, available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion: GP1569 epsA-gfp (spc)

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1526 epsA-FLAG 3x spc available in Stülke lab

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage