tkmA

tkmA
168

transmembrane modulator of PtkA activity, activates PtkA autophosphorylation and substrate phosphorylation

Locus
BSU_36260
Molecular weight
26.49 kDa
Isoelectric point
4.61
Protein length
248 aaSequenceBlast
Gene length
747 bpSequenceBlast
Function
control of protein tyrosine phosphorylation
Product
modulator of PtkA activity
Essential
no
Synonyms
tkmA, ywqC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3944 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,732,525  3,733,271
Phenotypes of a mutant
the mutant exhibits a defect in biofilm formation, pronounced on LBGM medium, weak of MSgg medium PubMed, this can be compensated for by overexpression of ''epsA'' or by mutations that reduce the expression of SinR PubMed
The protein
Catalyzed reaction/ biological activity
transmembrane activation of PtkA protein tyrosine kinase activity
Protein family
CapA family (with CapA and EpsA, according to UniProt)
CpsC/CapA family (with EpsA, according to UniProt)
Structure
P96715 (AlphaFold)
Paralogous protein(s)
EpsA
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
tkmA-ptkA-ptpZ-ugd
Description
PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
DegU: activation, DegU-P, PubMed, in degU regulon
AbrB: repression, PubMed, in abrB regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

tkmA→ugd

2025-03-17 18:24:39

ghost

105

c2e9da4a42fde0cc0e5b798663a12ae407bf7d28

23621A956181A8BE93792267290B609197EA8EF4

Biological materials
Mutant
MGNA-A034 (ywqC::erm), available at the NBRP B. subtilis, Japan
GP1566 (spc) PubMed, available in Jörg Stülke's lab
GP1567 ''epsA::aphA3 tkmA''::spc PubMed, available in Jörg Stülke's lab
BKE36260 (tkmA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATACCTCCAAATCTCT,  downstream forward: _UP4_TCTGAAAAAACGGGGAGTGG
BKK36260 (tkmA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATACCTCCAAATCTCT,  downstream forward: _UP4_TCTGAAAAAACGGGGAGTGG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1620 tkmA-FLAG 3x spc (based on pGP1331) available in Jörg Stülke's lab
References
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8DCA50478136628DD9E5D01E89609A686EED9F57

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Time of last update: 2025-03-31 15:31:10

Author of last update: Jstuelk