SigF

• Description: RNA polymerase forespore-specific (early) sigma factor SigF
  
  

Gene name	sigF
Synonyms	spoIIAC
Essential	no
Product	RNA polymerase forespore-specific (early) sigma factor SigF
Function	transcription of sporulation genes (early forespore)
Gene expression levels in SubtiExpress: sigF
Interactions involving this protein in SubtInteract: SigF
Metabolic function and regulation of this protein in SubtiPathways: sigF
MW, pI	29 kDa, 5.078
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	spoVAA, spoIIAB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigF regulon, SigG regulon, SigH regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU23450

Database entries

• BsubCyc: BSU23450

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s): SigG

Genes controlled by SigF

dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigF-Fin (probably) PubMed
  • SigF-SpoIIAB
  • SigF-(RpoB-RpoC)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU23450

• Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)

• UniProt: P07860

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • dacF-spoIIAA-spoIIAB-sigF
  • spoIIAA-spoIIAB-sigF

• Expression browser: sigF PubMed

• Sigma factor:
  • dacF: SigF, SigG
  • spoIIAA: SigH

• Regulation:
  • repressed by casamino acids PubMed
  • dacF: expressed during sporulation
  • spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)