DhbB

• Description: isochorismatase
  
  

Gene name	dhbB
Synonyms
Essential	no
Product	isochorismatase
Function	biosynthesis of the siderophore bacillibactin
Gene expression levels in SubtiExpress: dhbB
Metabolic function and regulation of this protein in SubtiPathways: DhbB
MW, pI	34 kDa, 4.429
Gene length, protein length	936 bp, 312 aa
Immediate neighbours	dhbF, dhbE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

acquisition of iron, iron metabolism

This gene is a member of the following regulons

AbrB regulon, Fur regulon

The gene

Basic information

• Locus tag: BSU31970

Database entries

• BsubCyc: BSU31970

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isochorismate + H₂O = 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to Swiss-Prot)

• Protein family: isochorismatase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU31970

• Structure:

• UniProt: P45743

• KEGG entry: [3]

• E.C. number: 3.3.2.1

Expression and regulation

• Operon: dhbA-dhbC-dhbE-dhbB-dhbF PubMed

• Expression browser: dhbB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by iron starvation (Fur) PubMed
  • strongly repressed in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 13865 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 4986 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: