dhbA

dhbA
168

2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

Locus
BSU_32000
Molecular weight
27.34 kDa
Isoelectric point
5.31
Protein length
261 aaSequenceBlast
Gene length
786 bpSequenceBlast
Function
biosynthesis of the siderophore bacillibactin
Product
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Essential
no
E.C.
1.3.1.28
Synonyms
dhbA, entA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,291,511  3,292,296
Phenotypes of a mutant
defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium PubMed
defective in sporulation timing PubMed
The protein
Catalyzed reaction/ biological activity
(2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + NAD+ --> 2,3-dihydroxybenzoate + H+ + NADH (according to UniProt)
Protein family
Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)
Structure
2FWM (PDB) (from E. coli, 42% identity) PubMed
P39071 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ
Description
PubMed
Regulation
induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
expression is increased in the presence of pulchimerrin PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
Fur: repression, PubMed, in fur regulon
AbrB: repression, PubMed, in abrB regulon
Kre: repression, in kre regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigI: sigma factor, PubMed, in sigI regulon
Additional information
the ''dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre'' mutant PubMed
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dhbA→ybdZ

2025-03-27 01:57:11

Jstuelk

147

875CBC1C026244834E2EFBC1140499985C0AB89D

CCA159D50C58E2078C51F543EDA686D77F6612EE

Biological materials
Mutant
BKE32000 (dhbA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATCAATTCCTTTCT,  downstream forward: _UP4_TAACTGAATTTAAAGGAGGT
BKK32000 (dhbA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATCAATTCCTTTCT,  downstream forward: _UP4_TAACTGAATTTAAAGGAGGT
LacZ fusion
pGP3594 (based on pAC7), available in Jörg Stülke's lab
References
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42C8D303AABFA2A9706295A0F90B0B49F854B0BF

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Time of last update: 2025-03-31 02:07:27

Author of last update: Jstuelk