Difference between revisions of "DnaE"
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Revision as of 14:17, 16 May 2013
- Description: DNA polymerase III (alpha subunit), part of the replisome
Gene name | dnaE |
Synonyms | |
Essential | yes PubMed |
Product | DNA polymerase III (alpha subunit) |
Function | DNA replication |
Gene expression levels in SubtiExpress: dnaE | |
Interactions involving this protein in SubtInteract: DnaE | |
MW, pI | 125 kDa, 6.02 |
Gene length, protein length | 3345 bp, 1115 aa |
Immediate neighbours | ytsJ, ytrH |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29230
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
- required for bacteriophage SPP1 replication PubMed
- Protein family: DnaE subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34623
- KEGG entry: [3]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Olivier Rannou, Emmanuelle Le Chatelier, Marilynn A Larson, Hamid Nouri, Bérengère Dalmais, Charles Laughton, Laurent Jannière, Panos Soultanas
Functional interplay of DnaE polymerase, DnaG primase and DnaC helicase within a ternary complex, and primase to polymerase hand-off during lagging strand DNA replication in Bacillus subtilis.
Nucleic Acids Res: 2013, 41(10);5303-20
[PubMed:23563155]
[WorldCat.org]
[DOI]
(I p)
Elena M Seco, John C Zinder, Carol M Manhart, Ambra Lo Piano, Charles S McHenry, Silvia Ayora
Bacteriophage SPP1 DNA replication strategies promote viral and disable host replication in vitro.
Nucleic Acids Res: 2013, 41(3);1711-21
[PubMed:23268446]
[WorldCat.org]
[DOI]
(I p)
Marjorie H Barnes, Michelle M Butler, George E Wright, Neal C Brown
Antimicrobials targeted to the replication-specific DNA polymerases of gram-positive bacteria: target potential of dnaE.
Infect Disord Drug Targets: 2012, 12(5);327-31
[PubMed:23017159]
[WorldCat.org]
[DOI]
(I p)
Andrew D Klocko, Jeremy W Schroeder, Brian W Walsh, Justin S Lenhart, Margery L Evans, Lyle A Simmons
Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork.
Mol Microbiol: 2011, 82(3);648-63
[PubMed:21958350]
[WorldCat.org]
[DOI]
(I p)
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290]
[WorldCat.org]
[DOI]
(P p)
Emmanuelle Le Chatelier, Olivier J Bécherel, Emmanuelle d'Alençon, Danielle Canceill, S Dusko Ehrlich, Robert P P Fuchs, Laurent Jannière
Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis.
J Biol Chem: 2004, 279(3);1757-67
[PubMed:14593098]
[WorldCat.org]
[DOI]
(P p)
E Dervyn, C Suski, R Daniel, C Bruand, J Chapuis, J Errington, L Jannière, S D Ehrlich
Two essential DNA polymerases at the bacterial replication fork.
Science: 2001, 294(5547);1716-9
[PubMed:11721055]
[WorldCat.org]
[DOI]
(P p)