mutL

mutL
168

sequence-unspecific endonuclease, DNA mismatch repair

Locus
BSU_17050
Molecular weight
70.25 kDa
Isoelectric point
5.58
Protein length
627 aaSequenceBlast
Gene length
1884 bpSequenceBlast
Function
DNA repair
Product
DNA mismatch repair protein, sequence-unspecific endonuclease
Essential
no
Synonyms
mutL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0323 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,778,337  1,780,220
Phenotypes of a mutant
60-fold increased mutation rate PubMed
a ''rnhB mutS-mutL'' mutant is not viable PubMed
The protein
Protein family
DNA mismatch repair mutL/hexB family (single member, according to UniProt)
Structure
3KDK (PDB) (C-terminal domain) PubMed
6E8D (PDB) (complexed with DnaN) PubMed
P49850 (AlphaFold)
Localization
forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed
Expression and Regulation
Operons
Genes
mutS-mutL
Description
PubMed
Additional information
A ncRNA is predicted between 'mutL' and 'ymzD' PubMed
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mutS→mutL

2025-03-10 04:21:34

ghost

105

904eecb53f1d0f2bcd0f561f80d88dc1faed6295

AD03305D7E5C23BD084F20F5C6F6EAC492F5C9FB

Biological materials
Mutant
GP1190 (mutS-mutL::aphA3), available in Jörg Stülke's lab PubMed
1A833 ( mutL::spec), PubMed, available at Bacillus Genetic Stock Center
BKE17050 (mutL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTGCCACATTCATCACCC,  downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT
BKK17050 (mutL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTGCCACATTCATCACCC,  downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT
References
Reviews
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Original Publications
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A86A013EDCE59D7EF84FB836A751E66C58CADF45

Page visits: 4612

Time of last update: 2025-04-02 00:45:04

Author of last update: Jstuelk