ytsJ

ytsJ
168

bifunctional malic/malolactic enzyme

Locus
BSU_29220
Molecular weight
43.51 kDa
Isoelectric point
5.05
Protein length
410 aaSequenceBlast
Gene length
1233 bpSequenceBlast
Function
malate utilization, balancing of the NADPH pool
Product
bifunctional malic/malolactic enzyme
Essential
no
E.C.
1.1.1.38
Synonyms
ytsJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,989,900 2,991,132
Phenotypes of a mutant
Poor growth with malate as single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
(S)-malate + NADP+ --> CO2 + NADPH + pyruvate PubMed
malate --> lactate + CO2 PubMed
Protein family
malic enzymes family (according to UniProt)
Cofactors
NADP+
Structure
1WW8 (PDB) (from Pyrococcus horikoshii, 46% identity, 63% similarity)
O34962 (AlphaFold)
Modification
phosphorylated on Arg-27 PubMed
Effectors of protein activity
in the presence of excess NADPH, the enzyme switches from oxidative pyruvate formation to non-oxidative lactate formation PubMed
Paralogous protein(s)
MalS, MleA
Localization
Cytoplasm (Homogeneous) PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
ytsJ
Description
PubMed
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ytsJ

2025-03-17 19:19:41

ghost

108

c76280c63d73af3c4e93f4b73f3fb7abab784b58

F8528F440486BCBD01D9FB7632E3FE592F580EBB

Genes
dnaE-ytsJ
Description
PubMed
Regulation
induced by DNA damage (LexA) PubMed
Regulatory mechanism
LexA: repression, PubMed, in lexA regulon
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dnaE→ytsJ

2025-04-01 20:26:19

Jstuelk

179

5807e5e50bd25f38dbc805171d3f14f5354cd973

F93681166D0A46D0BAA5D0678231F87A3D3BB25B

Biological materials
Mutant
MGNA-A176 (ytsJ::erm), available at the NBRP B. subtilis, Japan
GP612 (spc), available in Jrg Stlke's lab
GP1143 (spc), available in Jrg Stlke's lab
BKE29220 (ytsJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCCTAAACACTCCTT, downstream forward: _UP4_TAATTTTAATTCATTCCAAA
BKK29220 (ytsJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCCTAAACACTCCTT, downstream forward: _UP4_TAATTTTAATTCATTCCAAA
FLAG-tag construct
GP1131 (spc, based on pGP1331), available in Jrg Stlke's lab PubMed
GFP fusion
GP1432 (spc, based on pGP1870), available in Jrg Stlke's lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
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45229A538D5124B60CE05DDC8699B9BC3D5AD9FF

Page visits: 6158

Time of last update: 2025-04-02 03:02:12

Author of last update: Jstuelk