Difference between revisions of "DhbF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the siderophore bacillibactin
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the siderophore bacillibactin
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31960 dhbF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31960 dhbF]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 263 kDa, 4.737   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 263 kDa, 4.737   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybdZ]]'', ''[[dhbB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ybdZ]]'', ''[[dhbB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15186&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31960 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:dhbF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:dhbF_context.gif]]

Revision as of 13:45, 13 May 2013

  • Description: involved in 2,3-dihydroxybenzoate biosynthesis

Gene name dhbF
Synonyms
Essential no
Product unknown
Function biosynthesis of the siderophore bacillibactin
Gene expression levels in SubtiExpress: dhbF
MW, pI 263 kDa, 4.737
Gene length, protein length 7134 bp, 2378 aa
Immediate neighbours ybdZ, dhbB
Sequences Protein DNA Advanced_DNA
Genetic context
DhbF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DhbF expression.png
























Categories containing this gene/protein

acquisition of iron, iron metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, Fur regulon

The gene

Basic information

  • Locus tag: BSU31960

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-988 AND Ser-996 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947