SspE

• Description: small acid-soluble spore protein (major gamma-type SASP)
  
  

Gene name	sspE
Synonyms
Essential	no
Product	small acid-soluble spore protein (major gamma-type SASP)
Function	protection of spore DNA
Gene expression levels in SubtiExpress: sspE
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	9 kDa, 8.945
Gene length, protein length	252 bp, 84 aa
Immediate neighbours	fabL, ygaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon, YfhP regulon

The gene

Basic information

• Locus tag: BSU08660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gamma-type SASP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P07784

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon
  • mutY-fabL-sspE PubMed
  • fabL-sspE PubMed

• Expression browser: sspE PubMed

• Sigma factor: SigA PubMed, SigG PubMed

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S112 (no resistance), PubMed, available at BGSC
  • 1S113 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sandra M Ruzal, Patricia L Bustos, Carmen Sánchez-Rivas
"A la recherche" of functions for the spore protein SASP-E from Bacillus subtilis.
J Microbiol Biotechnol: 2013, 23(1);15-21
[PubMed:23314362] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

B Setlow, K A McGinnis, K Ragkousi, P Setlow
Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties.
J Bacteriol: 2000, 182(24);6906-12
[PubMed:11092849] [WorldCat.org] [DOI] (P p)

Hiroki Yamamoto, Masao Mori, Junichi Sekiguchi
Transcription of genes near the sspE locus of the Bacillus subtilis genome.
Microbiology (Reading): 1999, 145 ( Pt 8);2171-2180
[PubMed:10463184] [WorldCat.org] [DOI] (P p)

P Fajardo-Cavazos, F Tovar-Rojo, P Setlow
Effect of promoter mutations and upstream deletions on the expression of genes coding for small, acid-soluble spore proteins of Bacillus subtilis.
J Bacteriol: 1991, 173(6);2011-6
[PubMed:1900507] [WorldCat.org] [DOI] (P p)

D X Sun, P Stragier, P Setlow
Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.
Genes Dev: 1989, 3(2);141-9
[PubMed:2497051] [WorldCat.org] [DOI] (P p)

J M Mason, P Fajardo-Cavazos, P Setlow
Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis.
Nucleic Acids Res: 1988, 16(14A);6567-83
[PubMed:2456528] [WorldCat.org] [DOI] (P p)

R H Hackett, P Setlow
Cloning, nucleotide sequencing, and genetic mapping of the gene for small, acid-soluble spore protein gamma of Bacillus subtilis.
J Bacteriol: 1987, 169(5);1985-92
[PubMed:3106326] [WorldCat.org] [DOI] (P p)