mutY

mutY
168

A/G-specific adenine glycosylase, part of the error prevention oxidized guanine system (with MutM and RppH), releases adenines from 8-oxo-G:A mismatches

Locus
BSU_08630
Molecular weight
41.81 kDa
Isoelectric point
6.04
Protein length
369 aaSequenceBlast
Gene length
1110 bpSequenceBlast
Function
DNA repair
Product
A/G-specific adenine glycosylase
Essential
no
E.C.
3.2.2.-
Synonyms
mutY, yfhQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1194 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
935,656 → 936,765
Phenotypes of a mutant
strongly reduced stationary phase mutagenesis PubMed
increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed
The protein
Catalyzed reaction/ biological activity
releases adenines from 8-oxo-G:A mismatches
binds to 8-oxoG DNA lesions PubMed
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site (according to UniProt)
Protein family
Nth/MutY family (with Nth, according to UniProt)
Domains
HhH domain (aa 108-137) (according to UniProt)
Cofactors
Fe-S cluster PubMed
Structure
1RRS (PDB) (complex with DNA, Geobacillus stearothermophilus)
O31584 (AlphaFold)
Expression and Regulation
Operons
Genes
mutY-fabL-sspE
Description
PubMed
Regulation
expression is constitutive throughout growth PubMed
Regulatory mechanism
YfhP: repression, in yfhP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigG: sigma factor, PubMed, in sigG regulon
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mutY→sspE

2025-03-10 18:54:39

ghost

121

3a7a008cdfb01ec3e3352da847749371e7eede7c

C3DBF827F212448AA1AC6115B8D2A94E11E4693A

Biological materials
Mutant
MGNA-C322 (yfhQ::erm), available at the NBRP B. subtilis, Japan
BKE08630 (ΔmutY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT,  downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC
BKK08630 (ΔmutY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTGTTTCTCCCCAGT,  downstream forward: _UP4_ATCTCGGCTGCTCCGTAAAC
References
Reviews
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Original Publications
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B62083CF18FC4C796D95B960CF62FA963FC26CDE

Page visits: 4779

Time of last update: 2025-03-31 19:53:06

Author of last update: Jstuelk