SigF

• Description: RNA polymerase forespore-specific (early) sigma factor SigF
  
  

Gene name	sigF
Synonyms	spoIIAC
Essential	no
Product	RNA polymerase forespore-specific (early) sigma factor SigF
Function	transcription of sporulation genes (late mother cell)
Interactions involving this protein in SubtInteract: SigF
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	29 kDa, 5.078
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	spoVAA, spoIIAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigF regulon, SigG regulon, SigH regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU23450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s): SigG

Genes controlled by SigF

dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigF-Fin (probably) PubMed
  • SigF-SpoIIAB
  • SigF-(RpoB-RpoC)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)

• UniProt: P07860

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • dacF-spoIIAA-spoIIAB-sigF
  • spoIIAA-spoIIAB-sigF

• Expression browser: sigF PubMed

• Sigma factor:
  • dacF: SigF, SigG
  • spoIIAA: SigH

• Regulation:
  • repressed by casamino acids PubMed
  • dacF: expressed during sporulation
  • spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

The SigF regulon

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed

Jin Hwan Do, Masao Nagasaki, Satoru Miyano
The systems approach to the prespore-specific activation of sigma factor SigF in Bacillus subtilis.
Biosystems: 2010, 100(3);178-84
[PubMed:20298743] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A feeding tube model for activation of a cell-specific transcription factor during sporulation in Bacillus subtilis.
Genes Dev: 2009, 23(8);1014-24
[PubMed:19390092] [WorldCat.org] [DOI] (I p)

Céline Karmazyn-Campelli, Lamya Rhayat, Rut Carballido-López, Sandra Duperrier, Niels Frandsen, Patrick Stragier
How the early sporulation sigma factor sigmaF delays the switch to late development in Bacillus subtilis.
Mol Microbiol: 2008, 67(5);1169-80
[PubMed:18208527] [WorldCat.org] [DOI] (I p)

Oleg A Igoshin, Margaret S Brody, Chester W Price, Michael A Savageau
Distinctive topologies of partner-switching signaling networks correlate with their physiological roles.
J Mol Biol: 2007, 369(5);1333-52
[PubMed:17498739] [WorldCat.org] [DOI] (P p)

Oleg A Igoshin, Chester W Price, Michael A Savageau
Signalling network with a bistable hysteretic switch controls developmental activation of the sigma transcription factor in Bacillus subtilis.
Mol Microbiol: 2006, 61(1);165-84
[PubMed:16824103] [WorldCat.org] [DOI] (P p)

Jonathan Dworkin, Richard Losick
Developmental commitment in a bacterium.
Cell: 2005, 121(3);401-9
[PubMed:15882622] [WorldCat.org] [DOI] (P p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Joanna Clarkson, Iain D Campbell, Michael D Yudkin
Efficient regulation of sigmaF, the first sporulation-specific sigma factor in B.subtilis.
J Mol Biol: 2004, 342(4);1187-95
[PubMed:15351644] [WorldCat.org] [DOI] (P p)

Karen Carniol, Tae-Jong Kim, Chester W Price, Richard Losick
Insulation of the sigmaF regulatory system in Bacillus subtilis.
J Bacteriol: 2004, 186(13);4390-4
[PubMed:15205443] [WorldCat.org] [DOI] (P p)

Karen Carniol, Patrick Eichenberger, Richard Losick
A threshold mechanism governing activation of the developmental regulatory protein sigma F in Bacillus subtilis.
J Biol Chem: 2004, 279(15);14860-70
[PubMed:14744853] [WorldCat.org] [DOI] (P p)

Margaret S Ho, Karen Carniol, Richard Losick
Evidence in support of a docking model for the release of the transcription factor sigma F from the antisigma factor SpoIIAB in Bacillus subtilis.
J Biol Chem: 2003, 278(23);20898-905
[PubMed:12676949] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Jennifer T Kemp, Adam Driks, Richard Losick
FtsA mutants of Bacillus subtilis impaired in sporulation.
J Bacteriol: 2002, 184(14);3856-63
[PubMed:12081956] [WorldCat.org] [DOI] (P p)

J Dworkin, R Losick
Differential gene expression governed by chromosomal spatial asymmetry.
Cell: 2001, 107(3);339-46
[PubMed:11701124] [WorldCat.org] [DOI] (P p)

Q Pan, D A Garsin, R Losick
Self-reinforcing activation of a cell-specific transcription factor by proteolysis of an anti-sigma factor in B. subtilis.
Mol Cell: 2001, 8(4);873-83
[PubMed:11684022] [WorldCat.org] [DOI] (P p)

P Fawcett, P Eichenberger, R Losick, P Youngman
The transcriptional profile of early to middle sporulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2000, 97(14);8063-8
[PubMed:10869437] [WorldCat.org] [DOI] (P p)

E A Campbell, S A Darst
The anti-sigma factor SpoIIAB forms a 2:1 complex with sigma(F), contacting multiple conserved regions of the sigma factor.
J Mol Biol: 2000, 300(1);17-28
[PubMed:10864495] [WorldCat.org] [DOI] (P p)

A Petersohn, S Engelmann, P Setlow, M Hecker
The katX gene of Bacillus subtilis is under dual control of sigmaB and sigmaF.
Mol Gen Genet: 1999, 262(1);173-9
[PubMed:10503549] [WorldCat.org] [DOI] (P p)

N King, O Dreesen, P Stragier, K Pogliano, R Losick
Septation, dephosphorylation, and the activation of sigmaF during sporulation in Bacillus subtilis.
Genes Dev: 1999, 13(9);1156-67
[PubMed:10323866] [WorldCat.org] [DOI] (P p)

N Frandsen, I Barák, C Karmazyn-Campelli, P Stragier
Transient gene asymmetry during sporulation and establishment of cell specificity in Bacillus subtilis.
Genes Dev: 1999, 13(4);394-9
[PubMed:10049355] [WorldCat.org] [DOI] (P p)

D A Garsin, L Duncan, D M Paskowitz, R Losick
The kinase activity of the antisigma factor SpoIIAB is required for activation as well as inhibition of transcription factor sigmaF during sporulation in Bacillus subtilis.
J Mol Biol: 1998, 284(3);569-78
[PubMed:9826499] [WorldCat.org] [DOI] (P p)

D A Garsin, D M Paskowitz, L Duncan, R Losick
Evidence for common sites of contact between the antisigma factor SpoIIAB and its partners SpoIIAA and the developmental transcription factor sigmaF in Bacillus subtilis.
J Mol Biol: 1998, 284(3);557-68
[PubMed:9826498] [WorldCat.org] [DOI] (P p)

I Bagyan, L Casillas-Martinez, P Setlow
The katX gene, which codes for the catalase in spores of Bacillus subtilis, is a forespore-specific gene controlled by sigmaF, and KatX is essential for hydrogen peroxide resistance of the germinating spore.
J Bacteriol: 1998, 180(8);2057-62
[PubMed:9555886] [WorldCat.org] [DOI] (P p)

K Pogliano, A E Hofmeister, R Losick
Disappearance of the sigma E transcription factor from the forespore and the SpoIIE phosphatase from the mother cell contributes to establishment of cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1997, 179(10);3331-41
[PubMed:9150232] [WorldCat.org] [DOI] (P p)

M Gomez, S M Cutting
Expression of the Bacillus subtilis spoIVB gene is under dual sigma F/sigma G control.
Microbiology (Reading): 1996, 142 ( Pt 12);3453-7
[PubMed:9004507] [WorldCat.org] [DOI] (P p)

P J Lewis, T Magnin, J Errington
Compartmentalized distribution of the proteins controlling the prespore-specific transcription factor sigmaF of Bacillus subtilis.
Genes Cells: 1996, 1(10);881-94
[PubMed:9077448] [WorldCat.org] [DOI] (P p)

A L Decatur, R Losick
Three sites of contact between the Bacillus subtilis transcription factor sigmaF and its antisigma factor SpoIIAB.
Genes Dev: 1996, 10(18);2348-58
[PubMed:8824593] [WorldCat.org] [DOI] (P p)

A Decatur, R Losick
Identification of additional genes under the control of the transcription factor sigma F of Bacillus subtilis.
J Bacteriol: 1996, 178(16);5039-41
[PubMed:8759874] [WorldCat.org] [DOI] (P p)

S Alper, A Dufour, D A Garsin, L Duncan, R Losick
Role of adenosine nucleotides in the regulation of a stress-response transcription factor in Bacillus subtilis.
J Mol Biol: 1996, 260(2);165-77
[PubMed:8764398] [WorldCat.org] [DOI] (P p)

L Duncan, S Alper, R Losick
SpoIIAA governs the release of the cell-type specific transcription factor sigma F from its anti-sigma factor SpoIIAB.
J Mol Biol: 1996, 260(2);147-64
[PubMed:8764397] [WorldCat.org] [DOI] (P p)

A Feucht, T Magnin, M D Yudkin, J Errington
Bifunctional protein required for asymmetric cell division and cell-specific transcription in Bacillus subtilis.
Genes Dev: 1996, 10(7);794-803
[PubMed:8846916] [WorldCat.org] [DOI] (P p)

L Duncan, S Alper, F Arigoni, R Losick, P Stragier
Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division.
Science: 1995, 270(5236);641-4
[PubMed:7570023] [WorldCat.org] [DOI] (P p)

C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342] [WorldCat.org] [DOI] (P p)

E J Harry, K Pogliano, R Losick
Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(12);3386-93
[PubMed:7768847] [WorldCat.org] [DOI] (P p)

R Schuch, P J Piggot
The dacF-spoIIA operon of Bacillus subtilis, encoding sigma F, is autoregulated.
J Bacteriol: 1994, 176(13);4104-10
[PubMed:8021191] [WorldCat.org] [DOI] (P p)

P J Lewis, S R Partridge, J Errington
Sigma factors, asymmetry, and the determination of cell fate in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1994, 91(9);3849-53
[PubMed:8171000] [WorldCat.org] [DOI] (P p)

S Alper, L Duncan, R Losick
An adenosine nucleotide switch controlling the activity of a cell type-specific transcription factor in B. subtilis.
Cell: 1994, 77(2);195-205
[PubMed:8168129] [WorldCat.org] [DOI] (P p)

D Foulger, J Errington
Effects of new mutations in the spoIIAB gene of Bacillus subtilis on the regulation of sigma F and sigma G activities.
J Gen Microbiol: 1993, 139(12);3197-203
[PubMed:8126438] [WorldCat.org] [DOI] (P p)

K T Min, C M Hilditch, B Diederich, J Errington, M D Yudkin
Sigma F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-sigma factor that is also a protein kinase.
Cell: 1993, 74(4);735-42
[PubMed:8358793] [WorldCat.org] [DOI] (P p)

L Duncan, R Losick
SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis.
Proc Natl Acad Sci U S A: 1993, 90(6);2325-9
[PubMed:8460142] [WorldCat.org] [DOI] (P p)

J J Wu, R Schuch, P J Piggot
Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase.
J Bacteriol: 1992, 174(15);4885-92
[PubMed:1629150] [WorldCat.org] [DOI] (P p)

K York, T J Kenney, S Satola, C P Moran, H Poth, P Youngman
Spo0A controls the sigma A-dependent activation of Bacillus subtilis sporulation-specific transcription unit spoIIE.
J Bacteriol: 1992, 174(8);2648-58
[PubMed:1556084] [WorldCat.org] [DOI] (P p)

D Sun, P Fajardo-Cavazos, M D Sussman, F Tovar-Rojo, R M Cabrera-Martinez, P Setlow
Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters.
J Bacteriol: 1991, 173(24);7867-74
[PubMed:1744043] [WorldCat.org] [DOI] (P p)

P Margolis, A Driks, R Losick
Establishment of cell type by compartmentalized activation of a transcription factor.
Science: 1991, 254(5031);562-5
[PubMed:1948031] [WorldCat.org] [DOI] (P p)

N Illing, J Errington
Genetic regulation of morphogenesis in Bacillus subtilis: roles of sigma E and sigma F in prespore engulfment.
J Bacteriol: 1991, 173(10);3159-69
[PubMed:1902463] [WorldCat.org] [DOI] (P p)

S R Partridge, D Foulger, J Errington
The role of sigma F in prespore-specific transcription in Bacillus subtilis.
Mol Microbiol: 1991, 5(3);757-67
[PubMed:1904527] [WorldCat.org] [DOI] (P p)

R Schmidt, P Margolis, L Duncan, R Coppolecchia, C P Moran, R Losick
Control of developmental transcription factor sigma F by sporulation regulatory proteins SpoIIAA and SpoIIAB in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1990, 87(23);9221-5
[PubMed:2123551] [WorldCat.org] [DOI] (P p)

J J Wu, M G Howard, P J Piggot
Regulation of transcription of the Bacillus subtilis spoIIA locus.
J Bacteriol: 1989, 171(2);692-8
[PubMed:2492512] [WorldCat.org] [DOI] (P p)

J Errington, J Mandelstam
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis.
J Gen Microbiol: 1986, 132(11);2967-76
[PubMed:3114419] [WorldCat.org] [DOI] (P p)

M D Yudkin
The sigma-like product of sporulation gene spoIIAC of Bacillus subtilis is toxic to Escherichia coli.
Mol Gen Genet: 1986, 202(1);55-7
[PubMed:3007937] [WorldCat.org] [DOI] (P p)