SpoIIAA

• Description: anti-anti-SigF
  
  

Gene name	spoIIAA
Synonyms
Essential	no
Product	anti-anti-SigF
Function	control of SigF activity
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	12 kDa, 5.855
Gene length, protein length	351 bp, 117 aa
Immediate neighbours	spoIIAB, dacF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sigma factors and their control, sporulation proteins, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, SigF regulon, SigG regulon, SigH regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU23470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: anti-sigma-factor antagonist family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-58 OR Ser-59) by SpoIIABPubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIIAB-SpoIIAA PubMed

• Localization:

Database entries

• Structure: 1TIL (complex with SpoIIAB, Geobacillus stearothermophilus), 1AUZ (NMR)

• UniProt: P10727

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • dacF-spoIIAA-spoIIAB-sigF
  • spoIIAA-spoIIAB-sigF

• Sigma factor:
  • dacF: SigF, SigG
  • spoIIAA: SigH

• Regulation: repressed by glucose (5.2-fold) PubMed, dacF: expressed during sporulation, spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism: spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Charles Moran, Emory University, NC, USA homepage