Pel

• Description: pectate lyase C
  
  

Gene name	pel
Synonyms
Essential	no
Product	pectate lyase C
Function	degradation of polygalacturonic acid
MW, pI	45 kDa, 8.421
Gene length, protein length	1260 bp, 420 aa
Immediate neighbours	yflT, yflS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: polysaccharide lyase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

• Structure: 2O1D (bound to trisaccharide), 1BN8

• Swiss prot entry: P39116

• KEGG entry: [3]

• E.C. number: 4.2.2.2

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repressed by glucose (3.8-fold) (CcpA) PubMed

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
2. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed