SigL

• Description: sigma factor of the RNA polymerase, Sigma-54, Sigma L
  
  

Gene name	sigL
Synonyms
Essential	no
Product	RNA polymerase sigma-54 factor (sigma-L)
Function	utilization of arginin, acetoin and fructose, required for cold adaptation
Gene expression levels in SubtiExpress: sigL
Interactions involving this protein in SubtInteract: SigL
MW, pI	49,5 kDa, 7.79
Gene length, protein length	1308 bp, 436 amino acids
Immediate neighbours	yvfH, yvfG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU34200

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

Database entries

• BsubCyc: BSU34200

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type

• Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family

• Paralogous protein(s):

Transcription factors activating transcription at SigL-dependent promoters

AcoR, BkdR, LevR, RocR, YplP

Extended information on the protein

• Kinetic information:

• Domains:
  • DNA binding domain (H-T-H motif) (324–343)
  • pron box domain (413–421)
  • 3 x Compositional bias domain (6–21),(32–53),(112–136)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigL-(RpoB-RpoC-RpoA) PubMed
  • SigL-AcoR, SigL-RocR, SigL-LevR, SigL-BkdR, SigL-YplP,

• Localization:

Database entries

• BsubCyc: BSU34200

• Structure: 5BYH (E. coli RNA polymerase containing Sigma-54) PubMed

• UniProt: P24219

• KEGG entry: [3]

Additional information

Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.

Transcription factors activating transcription at SigL-dependent promoters

Expression and regulation

• Operon: sigL (according to DBTBS)

• Expression browser: sigL PubMed

• Sigma factor:

• Regulation: repressed by glucose (CcpA) PubMed

• Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock PubMed

• Additional information:

Biological materials

• Mutant:
  • GP146 ( sigL::spc), available in Jörg Stülke's lab PubMed
  • 1A914 ( sigL::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage