ThiG

• Description: hydroxyethylthiazole phosphate biosynthesis
  
  

Gene name	thiG
Synonyms	yjbT
Essential	no
Product	thiamin thiazole synthase
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiG
Interactions involving this protein in SubtInteract: ThiG
Metabolic function and regulation of this protein in SubtiPathways: thiG
MW, pI	26 kDa, 4.718
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	thiS, thiF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU11690

Database entries

• BsubCyc: BSU11690

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: thiG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ThiS-ThiG PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11690

• Structure: 1XM3, 1TYG (complex with ThiS) PubMed

• UniProt: O31618

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tenA-tenI-thiO-thiS-thiG-thiF-thiD PubMed

• Expression browser: thiG PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 8895 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 19986 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)