SspE

• Description: small acid-soluble spore protein (major gamma-type SASP)
  
  

Gene name	sspE
Synonyms
Essential	no
Product	small acid-soluble spore protein (major gamma-type SASP)
Function	protection of spore DNA
Gene expression levels in SubtiExpress: sspE
Metabolic function and regulation of this protein in SubtiPathways: sspE
MW, pI	9 kDa, 8.945
Gene length, protein length	252 bp, 84 aa
Immediate neighbours	fabL, ygaB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon, YfhP regulon

The gene

Basic information

• Locus tag: BSU08660

Database entries

• BsubCyc: BSU08660

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gamma-type SASP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU08660

• Structure:

• UniProt: P07784

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon
  • mutY-fabL-sspE PubMed
  • fabL-sspE PubMed

• Expression browser: sspE PubMed

• Sigma factor: SigA PubMed, SigG PubMed

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S112 (no resistance), PubMed, available at BGSC
  • 1S113 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sandra M Ruzal, Patricia L Bustos, Carmen Sánchez-Rivas
"A la recherche" of functions for the spore protein SASP-E from Bacillus subtilis.
J Microbiol Biotechnol: 2013, 23(1);15-21
[PubMed:23314362] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

B Setlow, K A McGinnis, K Ragkousi, P Setlow
Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties.
J Bacteriol: 2000, 182(24);6906-12
[PubMed:11092849] [WorldCat.org] [DOI] (P p)

Hiroki Yamamoto, Masao Mori, Junichi Sekiguchi
Transcription of genes near the sspE locus of the Bacillus subtilis genome.
Microbiology (Reading): 1999, 145 ( Pt 8);2171-2180
[PubMed:10463184] [WorldCat.org] [DOI] (P p)

P Fajardo-Cavazos, F Tovar-Rojo, P Setlow
Effect of promoter mutations and upstream deletions on the expression of genes coding for small, acid-soluble spore proteins of Bacillus subtilis.
J Bacteriol: 1991, 173(6);2011-6
[PubMed:1900507] [WorldCat.org] [DOI] (P p)

D X Sun, P Stragier, P Setlow
Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.
Genes Dev: 1989, 3(2);141-9
[PubMed:2497051] [WorldCat.org] [DOI] (P p)

J M Mason, P Fajardo-Cavazos, P Setlow
Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis.
Nucleic Acids Res: 1988, 16(14A);6567-83
[PubMed:2456528] [WorldCat.org] [DOI] (P p)

R H Hackett, P Setlow
Cloning, nucleotide sequencing, and genetic mapping of the gene for small, acid-soluble spore protein gamma of Bacillus subtilis.
J Bacteriol: 1987, 169(5);1985-92
[PubMed:3106326] [WorldCat.org] [DOI] (P p)