Pel

• Description: pectate lyase C
  
  

Gene name	pel
Synonyms
Essential	no
Product	pectate lyase C
Function	degradation of polygalacturonic acid
Gene expression levels in SubtiExpress: pel
MW, pI	45 kDa, 8.421
Gene length, protein length	1260 bp, 420 aa
Immediate neighbours	yflT, yflS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, ComA regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU07560

Database entries

• BsubCyc: BSU07560

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to Swiss-Prot)

• Protein family: polysaccharide lyase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide), major constituent of the secretome PubMed

Database entries

• BsubCyc: BSU07560

• Structure: 2O1D (bound to trisaccharide), 1BN8

• UniProt: P39116

• KEGG entry: [3]

• E.C. number: 4.2.2.2

Expression and regulation

• Operon: pel PubMed

• Expression browser: pel PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (3.8-fold) (CcpA) PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CcpA: transcription repression
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)