DhbF

• Description: involved in 2,3-dihydroxybenzoate biosynthesis
  
  

Gene name	dhbF
Synonyms
Essential	no
Product	unknown
Function	biosynthesis of the siderophore bacillibactin
Gene expression levels in SubtiExpress: dhbF
MW, pI	263 kDa, 4.737
Gene length, protein length	7134 bp, 2378 aa
Immediate neighbours	ybdZ, dhbB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

acquisition of iron, iron metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, Fur regulon

The gene

Basic information

• Locus tag: BSU31960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-988 AND Ser-996 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane PubMed

Database entries

• Structure:

• UniProt: P45745

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dhbA-dhbC-dhbE-dhbB-dhbF PubMed

• Expression browser: dhbF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by iron starvation (Fur) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947