DnaE

• Description: DNA polymerase III (alpha subunit), part of the replisome
  
  

Gene name	dnaE
Synonyms
Essential	yes PubMed
Product	DNA polymerase III (alpha subunit)
Function	DNA replication
Gene expression levels in SubtiExpress: dnaE
Interactions involving this protein in SubtInteract: DnaE
MW, pI	125 kDa, 6.02
Gene length, protein length	3345 bp, 1115 aa
Immediate neighbours	ytsJ, ytrH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU29230

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication PubMed

• Protein family: DnaE subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-MutL PubMed
  • DnaE-MutS PubMed
  • DnaE-DnaN PubMed
  • DnaE-SsbA PubMed
  • DnaE-DnaC PubMed
  • DnaE-DnaG PubMed
  • DnaE-HolA PubMed
  • DnaE-PolC PubMed

• Localization:
  • forms foci, the foci dissappear after the formation of DNA mismatches in a MutS-dependent manner or after DNA replication arrest PubMed
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34623

• KEGG entry: [3]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon: dnaE (according to DBTBS)

• Expression browser: dnaE PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: