Difference between revisions of "SigL"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[CcpA regulon]]}} | {{SubtiWiki regulon|[[CcpA regulon]]}} | ||
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| + | =The [[SigL regulon]]= | ||
=The gene= | =The gene= | ||
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* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
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===[[Transcription factors activating transcription at SigL-dependent promoters]]=== | ===[[Transcription factors activating transcription at SigL-dependent promoters]]=== | ||
Revision as of 20:01, 10 December 2010
- Description: sigma factor of the RNA polymerase, Sigma-54, Sigma L
| Gene name | sigL |
| Synonyms | |
| Essential | no |
| Product | RNA polymerase sigma-54 factor (sigma-L) |
| Function | utilization of arginin, acetoin and fructose, required for cold adaptation |
| MW, pI | 49,5 kDa, 7.79 |
| Gene length, protein length | 1308 bp, 436 amino acids |
| Immediate neighbours | yvfH, yvfG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
transcription, sigma factors and their control
This gene is a member of the following regulons
The SigL regulon
The gene
Basic information
- Locus tag: BSU34200
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
- Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
- Paralogous protein(s):
Transcription factors activating transcription at SigL-dependent promoters
Extended information on the protein
- Kinetic information:
- Domains:
- DNA binding domain (H-T-H motif) (324–343)
- pron box domain (413–421)
- 3 x Compositional bias domain (6–21),(32–53),(112–136)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P24219
- KEGG entry: [3]
Additional information
Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.
Transcription factors activating transcription at SigL-dependent promoters
Expression and regulation
- Operon: sigL (according to DBTBS)
- Additional information:
Biological materials
- Mutant: GP146 (aphA3), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Reviews
M Buck, M T Gallegos, D J Studholme, Y Guo, J D Gralla
The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.
J Bacteriol: 2000, 182(15);4129-36
[PubMed:10894718]
[WorldCat.org]
[DOI]
(P p)
M J Merrick
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
Mol Microbiol: 1993, 10(5);903-9
[PubMed:7934866]
[WorldCat.org]
[DOI]
(P p)
Original publications
