SpoIIAB

• Description: anti-SigF/ protein serine kinase
  
  

Gene name	spoIIAB
Synonyms
Essential	no
Product	anti-SigF/ protein serine kinase
Function	control of SigF activity septation; phosphorylation and inactivation of SpoIIAA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	16 kDa, 4.383
Gene length, protein length	438 bp, 146 aa
Immediate neighbours	sigF, spoIIAA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification, sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon, SigH regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU23460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)

• Protein family: anti-sigma-factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIIE-SpoIIAB, RsbV-SpoIIAB PubMed, SpoIIAB-SigG PubMed, SpoIIAB-SigF, SpoIIAB-SpoIIAA PubMed

• Localization:

Database entries

• Structure: 1L0O (complex with SigF, Geobacillus stearothermophilus), 1TIL (complex with SpoIIAA, Geobacillus stearothermophilus)

• UniProt: P10728

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • dacF-spoIIAA-spoIIAB-sigF
  • spoIIAA-spoIIAB-sigF

• Sigma factor:
  • dacF: SigF, SigG
  • spoIIAA: SigH

• Regulation:
  • dacF: expressed during sporulation
  • spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism: spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage