Difference between revisions of "ThiF"
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiG]]'', ''[[thiD]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[thiG]]'', ''[[thiD]]'' | ||
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| − | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11700 | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11700 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:thiF_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:thiF_context.gif]] | ||
Revision as of 09:55, 14 May 2013
- Description: hydroxyethylthiazole phosphate biosynthesis
| Gene name | thiF |
| Synonyms | yjbU |
| Essential | no |
| Product | unknown |
| Function | biosynthesis of thiamine |
| Gene expression levels in SubtiExpress: thiF | |
| Metabolic function and regulation of this protein in SubtiPathways: Thiamin | |
| MW, pI | 36 kDa, 5.619 |
| Gene length, protein length | 1008 bp, 336 aa |
| Immediate neighbours | thiG, thiD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11700
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): MoeB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O31619
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Narasimhan Sudarsan, Smadar Cohen-Chalamish, Shingo Nakamura, Gail Mitchell Emilsson, Ronald R Breaker
Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine.
Chem Biol: 2005, 12(12);1325-35
[PubMed:16356850]
[WorldCat.org]
[DOI]
(P p)
David M Duda, Helen Walden, John Sfondouris, Brenda A Schulman
Structural analysis of Escherichia coli ThiF.
J Mol Biol: 2005, 349(4);774-86
[PubMed:15896804]
[WorldCat.org]
[DOI]
(P p)
Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704]
[WorldCat.org]
[DOI]
(P p)
A S Pang, S Nathoo, S L Wong
Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.
J Bacteriol: 1991, 173(1);46-54
[PubMed:1898926]
[WorldCat.org]
[DOI]
(P p)
