Difference between revisions of "SigL"
Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin and fructose, <br/>required for cold adaptation | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin and fructose, <br/>required for cold adaptation | ||
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34200 sigL] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigL SigL] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigL SigL] | ||
Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvfH]]'', ''[[yvfG]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvfH]]'', ''[[yvfG]]'' | ||
|- | |- | ||
− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34200 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34200 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34200 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:sigL_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:sigL_context.gif]] |
Revision as of 13:53, 13 May 2013
- Description: sigma factor of the RNA polymerase, Sigma-54, Sigma L
Gene name | sigL |
Synonyms | |
Essential | no |
Product | RNA polymerase sigma-54 factor (sigma-L) |
Function | utilization of arginin, acetoin and fructose, required for cold adaptation |
Gene expression levels in SubtiExpress: sigL | |
Interactions involving this protein in SubtInteract: SigL | |
MW, pI | 49,5 kDa, 7.79 |
Gene length, protein length | 1308 bp, 436 amino acids |
Immediate neighbours | yvfH, yvfG |
Sequences | Protein DNA Advanced_DNA |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transcription, sigma factors and their control
This gene is a member of the following regulons
The SigL regulon
The gene
Basic information
- Locus tag: BSU34200
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
- Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
- Paralogous protein(s):
Transcription factors activating transcription at SigL-dependent promoters
Extended information on the protein
- Kinetic information:
- Domains:
- DNA binding domain (H-T-H motif) (324–343)
- pron box domain (413–421)
- 3 x Compositional bias domain (6–21),(32–53),(112–136)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P24219
- KEGG entry: [3]
Additional information
Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.
Transcription factors activating transcription at SigL-dependent promoters
Expression and regulation
- Operon: sigL (according to DBTBS)
- Sigma factor:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Reviews
Additional reviews: PubMed
M Buck, M T Gallegos, D J Studholme, Y Guo, J D Gralla
The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.
J Bacteriol: 2000, 182(15);4129-36
[PubMed:10894718]
[WorldCat.org]
[DOI]
(P p)
M J Merrick
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
Mol Microbiol: 1993, 10(5);903-9
[PubMed:7934866]
[WorldCat.org]
[DOI]
(P p)
Original publications
Additional publications: PubMed