Fla-che operon

The fla-che operon encodes major factors required for motility and chemotaxis

• The operon is expressed from promoters upstream of flgB, ylxF, and sigD:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • sigD-swrB PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed
  • sigD: SigA PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • reduced expression in the absence of PnpA (due to accumulation of SlrA) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed
  • DegU-P: transcription repression PubMed
  • DegU-P in complex with SwrA: transcription activation PubMed

Important publications on the expression and regulation of the fla-che operon

Bo Liu, Daniel B Kearns, David H Bechhofer
Expression of multiple Bacillus subtilis genes is controlled by decay of slrA mRNA from Rho-dependent 3' ends.
Nucleic Acids Res: 2016, 44(7);3364-72
[PubMed:26857544] [WorldCat.org] [DOI] (I p)

Qutaiba O Ababneh, Jennifer K Herman
CodY Regulates SigD Levels and Activity by Binding to Three Sites in the fla/che Operon.
J Bacteriol: 2015, 197(18);2999-3006
[PubMed:26170408] [WorldCat.org] [DOI] (I p)