Dat

• Description: D-alanine aminotransferase

(Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt)

Gene name	dat
Synonyms	yheM
Essential	no
Product	D-alanine aminotransferase
Function	peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Cell wall, Cell wall
MW, pI	31 kDa, 4.871
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yheN, nhaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09670

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)

• Swiss prot entry: O07597

• KEGG entry: [2]

• E.C. number: 2.6.1.21

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed