dat

dat
168

Locus
BSU_09670
Molecular weight
31.03 kDa
Isoelectric point
4.87
Protein length
282 aaSequenceBlast
Gene length
849 bpSequenceBlast
Function
peptidoglycan precursor biosynthesis
Product
D-alanine aminotransferase
Essential
no
E.C.
2.6.1.21
Synonyms
dat, yheM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,041,994  1,042,842
Phenotypes of a mutant
a alaT dat double mutant is auxotrophic for alanine PubMed
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + D-alanine --> D-glutamate + pyruvate (according to UniProt)
Protein family
Class-IV pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP (according to UniProt)
Structure
1G2W (PDB) (E177S Mutant, Geobacillus stearothermophilus, 43% identity)
O07597 (AlphaFold)
Expression and Regulation
Operons
Genes
dat
Description
PubMed
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dat

2025-03-14 18:54:49

Jstuelk

104

9225c8cb8edade57d7f510ef741ee576207c91fd

9E99DB119CB5BA95A086D43EA38CD64662862192

Biological materials
Mutant
MGNA-B489 (yheM::erm), available at the NBRP B. subtilis, Japan
BKE09670 (dat::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC,  downstream forward: _UP4_TAAATGAACGAAGAAAAGAG
BKK09670 (dat::kan  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC,  downstream forward: _UP4_TAAATGAACGAAGAAAAGAG
References
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Time of last update: 2025-04-03 04:54:12

Author of last update: Jstuelk