LiaR

• Description: two-component response regulator, regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin
  
  

Gene name	liaR
Synonyms	yvqC
Essential	no
Product	two-component response regulator
Function	regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin
MW, pI	22 kDa, 4.956
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	gerAC, liaS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33080

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O32197

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: liaI-liaH-liaG-liaF-liaS-liaR

liaS-liaR

• Sigma factor: SigA

• Regulation: liaI: induced by bacitracin (LiaR)
  • repressed during logrithmic growth (AbrB) PubMed

liaS: constitutive

• Regulatory mechanism: LiaR: transcription activation
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Thorsten Mascher, Karlsruhe Institute of Technology, Germany link

References

Sina Jordan, Eva Rietkötter, Mark A Strauch, Falk Kalamorz, Bronwyn G Butcher, John D Helmann, Thorsten Mascher
LiaRS-dependent gene expression is embedded in transition state regulation in Bacillus subtilis.
Microbiology (Reading): 2007, 153(Pt 8);2530-2540
[PubMed:17660417] [WorldCat.org] [DOI] (P p)

Sina Jordan, Anja Junker, John D Helmann, Thorsten Mascher
Regulation of LiaRS-dependent gene expression in bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system.
J Bacteriol: 2006, 188(14);5153-66
[PubMed:16816187] [WorldCat.org] [DOI] (P p)

Mélanie A Hamon, Nicola R Stanley, Robert A Britton, Alan D Grossman, Beth A Lazazzera
Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis.
Mol Microbiol: 2004, 52(3);847-60
[PubMed:15101989] [WorldCat.org] [DOI] (P p)

1. Jordan, S., A. Junker, J. D. Helmann, and T. Mascher. 2006. Regulation of LiaRS-dependent gene expression in Bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system. J. Bacteriol. 188: 5153-5166. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed