FliF

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  • Description: flagellar basal-body M-ring protein, membrane anchor of the basal body

Gene name fliF
Synonyms
Essential no
Product flagellar basal-body M-ring protein
Function movement and chemotaxis
Gene expression levels in SubtiExpress: fliF
Interactions involving this protein in SubtInteract: FliF
MW, pI 59 kDa, 4.37
Gene length, protein length 1608 bp, 536 aa
Immediate neighbours fliE, fliG
Sequences Protein DNA DNA_with_flanks
Genetic context
FliF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FliF expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16210

Phenotypes of a mutant

  • mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
  • no secretion of FlgM, permanent inhibition of SigD PubMed
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: fliF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 68 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 595 PubMed

Biological materials

  • Mutant:
    • DS7080 (marker-less in NCIB3610) PubMed
    • TB192 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB208 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References