FlgE

• Description: flagellar hook protein
  
  

Gene name	flgE
Synonyms	flgG
Essential	no
Product	flagellar hook protein
Function	movement and chemotaxis
Gene expression levels in SubtiExpress: flgE
MW, pI	27 kDa, 4.734
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	flgD, ylzI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16290

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16290

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
• lack of SigD-dependent gene expression (no hag expression), no motility PubMed

• Protein family: flagella basal body rod proteins family (according to Swiss-Prot)

• Paralogous protein(s): FlhO, FlhP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: bacterial flagellum basal body (according to Swiss-Prot), extracellular (no signal peptide) PubMed, membrane associated PubMed

Database entries

• BsubCyc: BSU16290

• Structure:

• UniProt: P23446

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon (see there for regulation)
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: flgE PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1780 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3035 PubMed

Biological materials

• Mutant:
  • DS4681 (marker-less in NCIB3610) PubMed
  • TB187 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB203 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: