CheB

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  • Description: MCP-glutamate methylesterase / two-component response regulator-like

Gene name cheB
Synonyms cheL
Essential no
Product MCP-glutamate methylesterase / two-component response regulator-like
Function motility and chemotaxis
Gene expression levels in SubtiExpress: cheB
Interactions involving this protein in SubtInteract: CheB
MW, pI 38 kDa, 8.119
Gene length, protein length 1071 bp, 357 aa
Immediate neighbours flhG, cheA
Sequences Protein DNA DNA_with_flanks
Genetic context
CheB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheB expression.png















Categories containing this gene/protein

motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16420

Phenotypes of a mutant

  • not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on a Asp residue by CheA PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:
    • in minimal medium, CheB is present with 2,100 +/- 400 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 92 PubMed

Biological materials

  • Mutant:
    • DS7306 (marker-less in NCIB3610) PubMed
    • TB19 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB213 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications