SpsK

• Description: dTDP -4-dehydrorhamnose reductase, spore coat polysaccharide synthesis
  
  

Gene name	spsK
Synonyms	ipa-73d
Essential	no
Product	dTDP -4-dehydrorhamnose reductase
Function	rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsK
MW, pI	31 kDa, 6.081
Gene length, protein length	849 bp, 283 aa
Immediate neighbours	spsL, spsJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU37820

Database entries

• BsubCyc: BSU37820

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: dTDP-4-dehydrorhamnose reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37820

• Structure: 3SC6 (dTDP-4-dehydrorhamnose reductase (rfbD) from Bacillus anthracis str. Ames in complex with NADP; 42% identity, 72% similarity)

• UniProt: P39631

• KEGG entry: [3]

• E.C. number: 1.1.1.133

Expression and regulation

• Operon: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL PubMed

• Expression browser: spsK PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Giuseppina Cangiano, Teja Sirec, Cristina Panarella, Rachele Isticato, Loredana Baccigalupi, Maurilio De Felice, Ezio Ricca
The sps Gene Products Affect the Germination, Hydrophobicity, and Protein Adsorption of Bacillus subtilis Spores.
Appl Environ Microbiol: 2014, 80(23);7293-302
[PubMed:25239894] [WorldCat.org] [DOI] (I p)

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)