ScoC

• Description: transcriptional repressor of genes expressed in the transition phase
  
  

Gene name	scoC
Synonyms	hpr, catA
Essential	no
Product	transcriptional repressor (MarR family)
Function	transition state regulator
Gene expression levels in SubtiExpress: scoC
Interactions involving this protein in SubtInteract: ScoC
Regulation of this protein in SubtiPathways: scoC
MW, pI	23 kDa, 5.188
Gene length, protein length	609 bp, 203 aa
Immediate neighbours	yhaI, yhaH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, transition state regulators, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, SalA regulon, SenS regulon

The gene

Basic information

• Locus tag: BSU09990

Database entries

• BsubCyc: BSU09990

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-3 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SinR-ScoC

• Localization:

Database entries

• BsubCyc: BSU09990

• Structure: 2FXA

• UniProt: P11065

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: scoC PubMed

• Expression browser: scoC PubMed

• Sigma factor:

• Regulation:
  • expressed upon transition into the stationary phase (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription activation PubMed
  • SenS: positive regulation PubMed
  • SalA: negative regulation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 217 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 495 PubMed

Biological materials

• Mutant: Strain OM213 of Ogura MA, et al PubMed, genotype trpC2 scoC::pMutin-scoC leuC7, available as BGSC 1A918
  • 1A918 ( scoC::erm), PubMed, available at BGSC

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: