RnhB

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  • Description: RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication

Gene name rnhB
Synonyms rnh
Essential no
Product Mn2+-dependent RNase HII
Function endonucleolytic cleavage of RNA in
RNA-DNA hybrid molecules
Gene expression levels in SubtiExpress: rnhB
MW, pI 28 kDa, 5.518
Gene length, protein length 765 bp, 255 aa
Immediate neighbours rbgA, ylqG
Sequences Protein DNA DNA_with_flanks
Genetic context
RnhB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RnhB expression.png















Categories containing this gene/protein

DNA replication, Rnases

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
    • removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication PubMed
  • Protein family: RNase HII family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number: 3.1.26.4.

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector: for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications