SpsI

• Description: glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis
  
  

Gene name	spsI
Synonyms	ipa-71d
Essential	no
Product	glucose-1-phosphate thymidylyltransferase
Function	rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsI
MW, pI	27 kDa, 5.385
Gene length, protein length	738 bp, 246 aa
Immediate neighbours	spsJ, spsG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU37840

Database entries

• BsubCyc: BSU37840

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • alpha-D-glucose-1-phosphate + dTTP --> dTDP-glucose PubMed

• Protein family: glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37840

• Structure:

• UniProt: P39629

• KEGG entry: [3]

• E.C. number: 2.7.7.24

Expression and regulation

• Operon: spsA-spsB-spsC-spsD-spsF-spsG-spsI-spsJ-spsK-spsL PubMed

• Expression browser: spsI PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Giuseppina Cangiano, Teja Sirec, Cristina Panarella, Rachele Isticato, Loredana Baccigalupi, Maurilio De Felice, Ezio Ricca
The sps Gene Products Affect the Germination, Hydrophobicity, and Protein Adsorption of Bacillus subtilis Spores.
Appl Environ Microbiol: 2014, 80(23);7293-302
[PubMed:25239894] [WorldCat.org] [DOI] (I p)

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)